BioToSkin offers a range of in vitro tests to support your claims.
Objectification studies of anti-aging claims make it possible to quantify the anti-aging effect of the active ingredient and finished formulation. Anti-aging studies can also be coupled with an objectification of antioxidant effects.
To help our customers in their claims we have developed the Glasbox+® system.
We also offer other studies such as measuring the booster effect of the production of molecules responsible for firmness (synthesis of collagen, elastin, hyaluronic acid) and ‘skin hydration.
For each in vitro study, we offer different cell types (fibroblast, keratinocyte, melanocyte, mesenchymal, etc.) from different anatomical parts, whether common (abdomen, scalp, thigh) or more marginal (eyelid, lip).
Wound healing: Incucyte
Objectification studies of healing effect claims make it possible to quantify the restorative effect of the active ingredient and finished formulation.
We primarily offer a protocol based on creating standarised wounds on cell monolayers using the incucyte S3 system (Sartorius), allowing monitoring of healing by imaging.
BioToSkin offers a wide range of services to validate the anti-inflammatory claims of products or formulations. For example, it is possible to measure the production of the molecule responsible for the inflammatory process (TNF alpha, IL1, IL8, HBD2, PGE2, etc.).
Anti-inflammatory claim studies can also be objectified using an ex-vivo model via skin microdialysis.
Depigmenting / Lightening
Objectification studies of lightening effect claims make it possible to quantify the depigmenting effect of the active ingredient and finished formulation.
We mainly offers protocols based on melanin quantification as well as a method for studying melanosome transfer. The latter makes it possible to validate the effect of the active ingredient via the inhibition of melanosome transfer.
Skin barrier repair
The skin barrier, essentially represented by the stratum corneum, plays an essential role in maintaining the hydration of the skin and the body. In addition, it also plays a protective role against various pathogens or chemicals or physical attacks.
BioToSkin offers the possibility to assess the effectiveness in terms of restoring or preserving the skin barrier of an active ingredient or formulation.
To do this, we use a skin explant model maintained in survival with measurements of TEWL (Trans Epidermal Water Loss) using a Tewameter (Courage+Khazaka) as well as hydration of the stratum corneum by corneometry using a Corneometer (Courage+Khazaka) after treatment of the skin explants with an active principle or a cosmetic formulation.
It is also possible to assess the repair of the skin barrier by histological monitoring by highlighting the morphology (Masson’s trichrome), the expression of epidermal differentiation markers (filaggrin, involucrin etc.) and any others at the same time.
Finally, we also offer the possibility of evaluating the repair of the skin barrier on models of burned skin as well as dry skin.